However, the fixation is non-reversible and it gives the best overall cytoplasmic and nuclear detail. Glutaraldehyde causes deformation of alpha-helix structure, so it is usually not a good choice for immunocytochemistry. It must be made from a powder no more than 1 week prior to use.ģ. Penetration is faster than formalin or glutaraldehyde. Paraformaldehyde is a purer version of formalin and is often used in its place as a superior fixative. It does not harm the structure of the protein greatly, usually antigenicity is not lost.Ģ. The standard solution is 10% neutral-buffered formalin. Aldehydes: Aldehydes fix samples by cross-linking proteins, especially between lysine residues.ġ. Check literature for optimum fixation methods for your specimen. A variety of fixatives is available for use depending on the type of tissue present and the features to be demonstrated. ![]() The purpose of fixation preserves the structure of the tissue permanently in as life-like a state as possible.įixation should be carried out as soon as possible after the removal of the tissues or (if possible) in-vivo or soon after death (in the case of autopsy). ![]() Without question, the most important step to viewing biological tissue is fixation. The study of histology is to understand the microanatomy of cells, tissues, and organs and also to learn about their functions in structural terms.
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